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In biochemical assays, an SCF E3 ligase complex containing Fbxo40 directly ubiquitinates IRS1, and this activity is enhanced by increased tyrosine phosphorylation of IRS1

In all cases, the images shown in the Figures are representative, and the outcome of the experiments qualitatively apparent ( e.g ., in showing the presence and extent of ectopic Cut expression)

2.2 Production and purification of carnitine monooxygenase The individual subunits CntA and CntB of carnitine monooxygenase were produced and purified as described elsewhere (Massmig et al., 2020)

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Periyasamy P, Singh S, Oladapo A, Kannan M, Buch S